HPLC quantitative analysis of protocatechuic acid contents in 11 Phellinus mushroom species collected in Thailand

Abstract Phellinus mushrooms have been traditionally used for various medicinal purposes. Protocatechuic acid, which was previously reported to be a component in some Phellinus mushrooms, has some pharmacological effects. This study aimed to validate a HPLC method for the quantitative analysis of the protocatechuic acid contents in the extracts from different Phellinus mushroom species collected in Thailand. HPLC was carried out using a C18 column and the gradient mobile phases of 0.1% formic acid in water and 0.1% formic acid in acetonitrile. Method validation was performed to assure the linearity, accuracy, precision, limit of detection and limit of quantitation of the analytical method. The linearity range of protocatechuic acid was 1 - 10 µg/ml. The average recovery was 104.16%. The method was shown to be precise with the RSD of repeatability and intermediate precision at less than 3%. The protocatechuic contents in 11 Phellinus mushrooms were in the range of less than 0.0099 - 0.4121 %w/w of the extract. The developed HPLC method was reliable and suitable for the quantitative analysis of protocatechuic acid content in Phellinus mushrooms.

Differences of body composition and physical strength among Japanese and Thai older adults living in Chiang Mai, Thailand: an inter-ethnic cross-sectional study

BACKGROUND@#The number of adults aged over 65 years is rapidly increasing in several Southeast Asian countries. Muscle mass decreases with age, leading to sarcopenia. The primary objective of this study was to determine whether differences exist in the body composition and physical strength, according to ethnicity, among community-dwelling Japanese and Thai older adults living in Chiang Mai Province, Thailand.@*METHODS@#A survey was conducted in February and March 2019. Japanese and Thai adults aged ≥ 60 years living in Chiang Mai Province were recruited through community clubs. Participants completed a self-administered questionnaire that enabled collection of data on age, sex, educational background, marital status, annual income, current medical conditions, smoking and alcohol consumption, and exercise habits. Measurements were collected on height, weight, body composition, blood pressure, hand grip, and walking speed for 6 m. Body composition was measured using a standing-posture 8-electrode multifrequency bioimpedance analysis analyzer. Hand grip of each hand was measured with the patient in the standing position using a digital grip dynamometer. Multivariable logistic regression was used to determine factors associated with skeletal muscle mass index (SMI).@*RESULTS@#Of the total 119 participants, 47 were Japanese (26 men, 21 women) and 72 were Thai (16 men, 56 women). The prevalence of a low SMI was 3/26 (12%), 1/21 (5%), 6/16 (38%), and 5/56 (9%) among Japanese men, Japanese women, Thai men, and Thai women, respectively. The prevalence of low muscle strength was 2/26 (8%), 2/21 (10%), 3/16 (19%), and 13/56 (23%) among Japanese men, Japanese women, Thai men, and Thai women, respectively. There were significant differences between ethnic groups in body mass index for both sexes, percentage body fat in women, SMI in men, and average grip strength in men. Ethnic group, sex, age, and body mass index were independent predictors of SMI.@*CONCLUSIONS@#Ethnicity had a clinically important effect on body composition and physical strength among older Japanese and Thai adults living in a similar environment.

Rebuilding Babel: finding common development solutions using cross-contextual comparisons of multidimensional well-being / Reconstruindo Babel: comparando o bem-estar multidimensional de diferentes contextos culturais em busca de soluções comuns para o desenvolvimento / Reconstruyendo Babel: la comparación del bienestar multidimensional de diferentes contextos culturales en búsqueda de soluciones comunes para el desarrollo

In this paper the theoretical tradition of coping strategies and capital portfolios is used as the basis for adaption and combination of existing methodologies to analyze well-being in rural households. Special attention is given to comparisons among different contexts. First we estimate a multidimensional measurement of poverty based on fuzzy logic for two areas of rural frontiers: Nang Rong, Thailand, and Altamira, in the Amazon Basin in Brazil. To enable a cross-contextual comparison we calculated a second estimate using a subset of shared measurements in the two areas. The findings suggest that the pattern of responses on a range of numerous key variables - including education, income and demographic dependency ratio - is robust for the model specification. It is concluded that comparative generalizations, useful in formulating cost-effective public policy interventions across contexts, could be satisfactorily identified in many situations. More generically, this approach provides researchers and policymakers with a framework for understanding the interaction of contexts with the subjective construction of well-being. The understanding of this interaction is useful for distinguishing stable corollaries of poverty from those that are volatile across contexts.

Com base na tradição teórica relativa a estratégias de sobrevivência e portfólios de capitais, propõe-se uma adaptação e combinação de metodologias existentes para análise do bem-estar em domicílios rurais, com especial atenção à comparação entre diferentes contextos. Em primeiro lugar, estimou-se uma medida multidimensional de pobreza, baseada em lógica nebulosa, para duas áreas de fronteira agrícola: Nang Rong, na Tailândia, e Altamira, no Brasil. Para que a comparação entre os contextos fosse possível, uma segunda estimativa foi obtida, utilizando-se um subconjunto das medidas presentes nas duas áreas de estudo. Os resultados sugerem que o padrão de resposta em relação a várias características-chave - por exemplo, educação, renda e razão de dependência demográfica - é robusto à especificação do modelo. Conclui-se que generalizações comparativas, úteis na formulação de políticas públicas que sejam custo-eficientes quanto à intervenção em contextos distintos, poderiam ser identificadas em diversas situações. Mais genericamente, a presente abordagem fornece aos pesquisadores e gestores de políticas um arcabouço que possibilite entender a interação do contexto com a construção subjetiva de bem-estar. A compreensão dessa interação é útil para distinguir corolários estáveis de pobreza daqueles que são voláteis em contextos distintos.

En base a la tradición teórica relativa a estrategias de supervivencia y carteras de capitales, se propone una adaptación y combinación de metodologías existentes para analizar el bienestar en viviendas rurales, con especial atención a la comparación entre diferentes contextos. En primer lugar, se estimó una medida multidimensional de pobreza, basada en lógica nebulosa, para dos áreas de frontera agrícola: Nang Rong, en Tailandia, y Altamira, en Brasil. Para que la comparación entre los contextos fuese posible, se llegó a una segunda estimación, utilizando un subconjunto de las medidas presentes en las dos áreas de estudio. Los resultados sugieren que el patrón de respuesta en relación a varias características clave - por ejemplo, educación, ingresos y razón de dependencia demográfica - es robusto en lo que concierne a la especificación del modelo. Se concluye que generalizaciones comparativas, útiles en la formulación de políticas públicas costo-eficientes en lo que se refiere a la intervención en contextos distintos, podrían identificarse en diversas situaciones. Más genéricamente, el presente abordaje ofrece a investigadores y gestores de políticas una base que posibilite entender la interacción del contexto con la construcción subjetiva de bienestar. La comprensión de esta interacción es útil para distinguir corolarios estables de pobreza de aquellos que son volátiles en contextos distintos.

Comprehensive intermaxillary tooth width proportion of Bangkok residents

Proper occlusion depends on the correct width ratio between upper and lower teeth, known as Bolton's ratio. In fact, this ratio can be calculated for each pair of teeth from the central incisor to the first permanent molar. This set of ratios, known as comprehensive cumulative percentage ratios (CPRs), can be used not only to determine which tooth or teeth have a tooth width discrepancy, but can also enable the partial graphical analysis of tooth width discrepancy when there is agenesis of certain permanent teeth. Although CPRs have been calculated for Caucasians, tooth width is known to vary depending on racial origin. Therefore, a test of differences between racial groups should be carried out. If these ratios of the Caucasians and Bangkokians are significantly different, the ratio of the Bangkokians is recommended. The objective of this study was to measure tooth size disproportion for Thai patients and to calculate a corresponding set of CPRs. Thirty-seven pairs of dental models were made from a group of Bangkok residents with normal occlusion. Mesiodistal tooth width was measured for each model. The intra- and inter-examiner measurement errors were ascertained as insignificant (p > 0.05). CPRs were then calculated and compared to those derived from other studies. Ten of thirteen CPRs were significantly different from corresponding values derived from Caucasians. We conclude that tooth width ratios vary between different racial groups, and therefore that these should be calculated specifically for each patient racial group.

The temporal variations of presumptive sudden death of Thai people in Singapore and Taiwan.

BACKGROUND: Sudden Unexplained Death Syndrome (SUDS) is the major cause ofsudden death in Thai adults, especially Thai migrant workers in Singapore and Taiwan. Temporal variations of sudden death of Thai people abroad are not well known. OBJECTIVE: To study the month, day and time of death of presumptive sudden death (PSD) in Singapore, Taiwan. MATERIAL AND METHOD: The authors reviewed the death certificates of Thai people who died in Singapore and Taiwan and previously SUDS reported cases form Singapore. The time, day and month ofPSD and Non-PSD deaths in Singapore and Taiwan were compared. RESULTS: From January 1994 to January 1995, 46 SUDS died in Singapore (gr A), from May 2000 to August 2002, 39 PSD died in Singapore (gr B), from January 1999 to May 2002, 100 presumptive or probable sudden unexplained death syndrome (PSUDS) died in Taiwan (gr C) and 254 Non-PSD death aboard (gr D) as controls. The annual SUDS/PSD death rates (per 100,000) in Singapore were 91.1 in 1994, 30.7 in 2001 and 33.5 in Taiwan in 2000. All but two SUDS/PSD cases were male. The mean age in gr A + B and C were 34.9 + 7.5 and 33.1 +/- 6.0 years old respectively. In gr. A, B and C, compared with gr D, Tuesday was the weekday of lowest SUDS/PSD death rate and Saturday was the highest. (p < 0.05). Time of death in gr B and C were peak during midnight to 8 a.m. and there was some trend of seasonal variation in occurrence of SUDS/PSD with the peak death rate in April and trough death rate in September; which is significantly different from gr D (1.49% vs 10.89% of all deaths, p < 0.01). CONCLUSION: The presented data demonstrate some temporal variations in SUDS/PSD death aboard. The sudden death of Thai people in Singapore and Taiwan may be more prevalent in the "work-to-rest" than "rest-to-work" periods.

The impact of overactive bladder, stress and mixed urinary incontinence on quality of life in Thai postmenopausal women.

OBJECTIVE: To assess the quality of life (QOL) of Thai postmenopausal women with overactive bladder (OAB), stress urinary incontinence (SUI) and mixed urinary incontinence (MUI). MATERIAL AND METHOD: A total of 420 Thai postmenopausal women (120 had SUI, 60 MUI, 120 OAB and 120 controls) were recruited QOL was assessed by the Thai version short form 36 (SF-36). RESULTS: There was no significant difference in the patients's characteristics. All domains of SF-36 in women with OAB, SUI and MUI were statistically significantly lower than the control group. OAB and MUI had lower SF-36 scores than SUI in all domains except the role of physical health. When compared between OAB and MUI, the women with MUI had lower SF-36 scores than OAB in all domains (except the physical functioning and general health). CONCLUSION: OAB, SUI and MUI had more significant impaired QOL than control. The author found a greater impact on QUL in OAB and MUI in Thai postmenopausal women.

Serum gamma-glutamyl transpeptidase and alkaline phosphatase of people in Khon Kaen, the northeastern Thailand.

Serum gamma-glytamyl transpeptidase (GGT) and alkaline phosphatase (ALP) of the normal population in Northeastern Thailand were measured. The median serum GGP and ALP levels of females was significantly lower than the values for males, i.e., 21 (range=9-260) versus 32 (range=9-459) and 73 (range= 20-428) versus 83 (range=13-171) U/L, respectively. Serum ALP level tended to increase with age in both male and female populations, whereas serum GGT level did not show any age dependence. Serum GGT and ALP levels of cigarette smokers were significantly higher than those of the non-smokers. Alcohol, coffee or tea drinking also resulted in higher serum GGT value whereas serum ALP values were not changed. Beta l nut chewing caused only lower serum GGT but not serum ALP values. The total population in Ban Fang district seemed to have lower serum GGT than those in Chonnabot district. The results from our study give the base line data of serum GGT and ALP in a Thai population. We also confirm the association of serum GGT with cigarette smoking, alcohol and coffee/tea drinking which are risk factors of cancer.

The first report of CCR5 delta 32 mutant in Thai injecting drug users.

CCR5, a chemokine receptor, is the principal coreceptor for macrophage-tropic HIV-1 which is the most important variant for viral transmission. It has been demonstrated that a homozygous genotype of a 32-bp deletion in CCR5 gene (delta32CCR5) shows a high degree of resistance to HIV-1 infection. To demonstrate that delta32CCR5 does exist in Thai natives, the CCR5 genotypes and allelic frequencies in 860 Thai injecting drug users (IDUs) were determined by PCR and DNA sequencing. Of these, six (0.7%) were CCR5/delta32CCR5 heterozygotes and no homozygote was found. The overall delta32CCR5 allelic frequency was 0.0035 and in HIV-1 seronegative (n = 490) and seropositive (n = 370) IDUs were 0.0051 and 0.0004, respectively, which were not significantly different (p = 0.3776). Here we report that the delta32CCR5 does exist in Thai IDUs as it is present in other human races. Such low allelic frequency may indicate that this mutation does not attribute a significant role in HIV-1 transmission in Thai IDUs.

Clinical effects of 17 beta-estradiol and norethisterone acetate in postmenopausal Thai women.

OBJECTIVE: To compare the climacteric symptoms, the bleeding patterns, side effects, endometrial thickness, serum follicular stimulating hormone (FSH) and estradiol level in postmenopause Thai women who received this drug and placebo. STUDY DESIGN: Double blind, randomized placebo controlled trial. MATERIAL AND METHOD: Sixty postmenopausal women attending the menopause clinic at Chulalongkorn Hospital from July, 1996 to December, 1996, were enrolled in the study. The patients were randomized to receive the placebo or drug (17 beta-estradiol 2 mg and norethisterone acetate 1 mg) continuously. After 12 months, 13 patients in the drug group were switched to have placebo and 13 patients in the placebo were switched to the drug group. Recording of patient characteristics, physical and gynecologic examination, pap smear, breast examination and mammogram, climacteric symptom scores, transvaginal ultrasonography, serum FSH and Estradiol level were performed prior to the study. Physical examinations, breast palpitations, measurement of body weight and blood pressure, climacteric symptom scores and side effects were repeated at 3, 6, 12, 18 months. Gynecologic examination, pap smear, serum FSH and estradiol, transvaginal ultrasonography, were repeated at 12 months. RESULTS: The women in the drug group had fewer climacteric symptoms than baseline after 6 months. The incidence of amenorrhea was 74.0 per cent at 12 months. The incidence of abnormal uterine bleeding (spotting and breakthrough bleeding) was 37.0 per cent at 3 months, 29.6 per cent at 6 months, 25.9 per cent at 12 months and 7.1 per cent at 18 months. The women in the drug group had fewer climacteric symptoms than baseline after 6 months. Breast tenderness was the most common side effect. The endometrial thickness decreased significantly in the drug group. Serum FSH decreased and serum estradiol increased significantly in the drug group. CONCLUSION: This combined regimen was more effective in relieving the climacteric symptoms in women who used the drug than those who used the placebo. There was a high incidence of amennorhoea after 12 months. But there was also a high frequency of abnormal bleeding in the first 3 months of treatment. Counseling on the bleeding pattern and common side effects should be conducted before starting the treatment to increase the compliance. This combined regimen proved to be a useful alternative for postmenopausal Thai women who prefer to avoid cyclic bleeding from sequential therapy.

Clinical training opportunities for Thai medical graduates in the United States.

There are many opportunities for a foreign medical graduate to receive graduate medical education in the US. It is unclear whether the chance is increasing or decreasing. Careful planning requires around a year in advance.

Knowledge and attitudes about AIDS: a comparative study of Thais involved in sexual occupations, university students and immigrants living in the United States.

Knowledge about AIDS among: (a) 89 individuals involved in prostitution related occupations, (b) 465 university students, and (c) 220 Thais living in the United States was assessed to further evaluate the efficacy of Thailand's AIDS educational programs. While the average number of items answered correctly by the three groups were not equivalent (p < 0.001), all participants possessed substantial factual AIDS knowledge. As predicted, those involved in prostitution had the least accurate information (92% correct). Contrary to initial hypothesizing, however, AIDS knowledge was identical (95%) for the samples of university students living in Thailand and Thais who have been exposed to intensive AIDS information campaigns in the United States. Taken together, these findings suggest that Thailand's AIDS educational efforts have achieved considerable success. Television, newspapers, and radio provided information for most participants while a few learned about AIDS from their physician or in educational institutions. A majority expressed interest in improving their understanding about AIDS. The extent of infection among prostitutes appears to be high with nearly one-quarter of those in the sexual occupations group reporting that they knew someone with the disease. Techniques to better inform prostitutes about AIDS are discussed and a common outcome instrument to measure AIDS knowledge and attitudes is recommended.

G-6-PD deficiency in the Pootai and the So communities in northeast Thailand.

The distribution of G-6-PD deficiency amongst two ethnic groups the Pootai and the So in northeast Thailand were studied. The prevalence of G-6-PD deficiency amongst the Pootai males was 9.7% while that amongst the So males was only 2.3%.